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FastANI is developed for fast alignment-free computation of whole-genome Average Nucleotide Identity (ANI).


  • FastANI on GitHub
  • Citation: If you use FastANI in your work, please cite:
    Jain, C., Rodriguez-R, L.M., Phillippy, A.M. et al. High throughput ANI analysis of 90K prokaryotic genomes reveals clear species boundaries. Nat Commun 9, 5114 (2018). https://doi.org/10.1038/s41467-018-07641-9.

Usage on Bridges-2

To see what versions of FastANI are available and if there is more than one, which is the default, along with some help, type

module spider fastani

To use FastANI, include a command like this in your batch script or interactive session to load the FastANI module: (note ‘module load’ is case-sensitive):

module load fastANI