Trinity, developed at the Broad Institute, represents a novel method for the efficient and robust de novo reconstruction of transcriptomes from RNA-Seq data. Trinity combines three independent software modules: Inchworm, Chrysalis, and Butterfly, applied sequentially to process large volumes of RNA-Seq reads. Trinity partitions the sequence data into many individual de Bruijn graphs, each representing the transcriptional complexity at at a given gene or locus, and then processes each graph independently to extract full-length splicing isoforms and to tease apart transcripts derived from paralogous genes.






To see what versions of Trinity are available type

module avail trinity

To see what other modules are needed, what commands are available and how to get additional help type

module help  trinity

To use Trinity, include a command like this in your batch script to load the Trinity module:

module load trinity


Be sure you also load any other modules needed, as listed by the module help trinity command.


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